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DNA microarray analysis revealed that GR5 is highly divergent; its genes were not sufficiently similar to be detected by microarray hybridization in any other Cronobacter strains.
A reference design was used for microarray hybridization in this study.
XL and CGL carried out the microarray hybridization in the 1st facility directed by CMC.
MHC carried out the microarray hybridization in the 2nd facility directed by GR.
All calculations are performed directly on the AffyBatch object and carried out separately for each particular microarray hybridization in a single-chip approach.
Double stranded cDNA was synthesized and followed by an in vitro transcription reaction to amplify aRNA incorporated with biotin labeling system for the microarray hybridization in triplicate.
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Similar considerations regarding cDNA length and distance from the 3' prime end apply to other workflows that rely on cDNA synthesis, such as microarray hybridizations, in cases where oligo-dT priming is used.
However, we also performed two additional microarray hybridizations in which 50 vs 180 ATU healthy fry, and 50 vs 180 ATU M74 fry, were compared.
LG performed sampling, library preparation, sequencing, bioinformatics analysis of the ESTs, microarray construction, microarray hybridizations, in situ hybridizations, interpretation of the data, and contributed to the overall conception, experimental design of the project and drafting of the manuscript.
Gene expression signatures of primary bursal B cells, pre-neoplastic and neoplastic lymphoma cells were collected by microarray hybridizations in a first attempt to identify genes up- or down-regulated during myc-induced B cell lymphoma development [ 8].
Utilizing our high performance DeGNServer, typical genome-scale gene networks involving 40,000~50,000 gene models could be constructed from expression data that consists of ~200 microarray hybridizations in less than 30 minutes.
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