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Microarray hybridization has been described in detail elsewhere [ 60].
Genotyping by microarray hybridization has proven to be challenging in species with complex genomes.
First, though it has been pointed out that the probes undergo folding (Binder et al., 2004), the contribution of this to microarray hybridization has not been estimated quantitatively.
Microarray hybridization has been used to study the global gene expression from many different kinds of tissues and cell lines [ 1- 4].
Gene expression profiling, based on microarray hybridization, has been successfully used for the identification of genes that are differentially expressed among RCC subtypes and in the search for new therapeutic targets [ 3– 6].
Microarray hybridization has been widely used to undertake genomic comparisons involving a great number of microorganisms [ 29] and previous work from our group has established precise criteria to employ this methodology to the study of Xf strains, with the aid of an Xf 9a5c biochip [ 30].
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Methods of sample preparation and microarray hybridization have been described previously [21].
RNA used for microarray hybridization had an RNA Integrity Number of >8.5.
RNA used for microarray hybridization had an average RNA Integrity Number of 8.2.
The LCM, RNA isolation, and microarray hybridization have been described in detail in previous work [ 14, 15].
Finally, we found that 12% of the genes recovered from our microarray hybridization have been described as genes differentially expressed between gastrula and syncytial blastoderm stages [ 26].
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