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The microarray hybridization experiment was carried out at Genotypic (Bangalore, India).
The bioinformatics search identified additional 16 motifs upstream of genes that were not detected during the DNA microarray hybridization experiment.
In addition, our microarray platform approach can simultaneously profile sense and antisense expression in one microarray hybridization experiment.
This analysis was designed to reveal the ability of RNA samples for the microarray hybridization experiment [ 28].
Since each single microarray hybridization experiment produces massive amounts of data, handling, processing and analyzing the data become challenging tasks and the application of bioinformatics is absolutely essential.
The microarray hybridization experiment included 3 biological replicates for each of the following conditions: control, 0.5, 2, and 8 mg/L, RDX (target concentrations).
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Microarray hybridization experiments using a tomato gene chip hybridized with potato RNA allowed the identification of known as well as novel genes that were differentially expressed during tuber cold storage in a single potato genotype.
All the S. pneumoniae study isolates were hybridized once against the TIGR4 reference strain and the microarray hybridization experiments were repeated for isolates which gave poor hybridization results.
Microarray hybridization experiments were performed by NimbleGen Systems using a duplicated dye-swap design.
Finally, the results obtained by qRT-PCR validate our microarray hybridization experiments, which were carried out with bacterial RNA extracted from three independent experiments.
In fact we might have missed those interesting homeobox genes if we had not performed both the SM RT-PCR and DNA microarray hybridization experiments.
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