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YH performed microarray hybridization, analyzed part of microarray and cDNA library datasets.
Each dataset consisted of several Affymetrix CEL-files, each one representing the raw data from one microarray hybridization.
Microarray hybridization was performed at 65°C for 17 h in Agilent's SureHyb hybridization chambers.
Labelled DNA was then genotyped using DNA microarray hybridization.
About 2 μg RNA was isolated from anthers and used for RNA labeling and microarray hybridization.
RNA isolation, cDNA synthesis, and microarray hybridization were performed according to previous protocols [45].
Microarray hybridization, washing, and staining were conducted following the standard procedure described in the GeneChip® Expression Analysis Technical Manual (Affymetrix).
The pooled RNA from TN1 and Kavya were used for the construction of a SSH cDNA library and microarray hybridization.
Microarray Hybridization and Original Data Normalization.
Affymetrix microarray hybridization signals were normalized with dChip [89].
All samples for microarray hybridization were processed at the Roche NimbleGen Service Laboratory.
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