Various PCR tests, microarray hybridization and DNA sequencing, revealed that residual Escherichia coli DNA from thermostable DNA polymerases was the cause of this cross-reaction.
Isolated RNA in DEPC-treated water was then immediately frozen at −80°C for 24 h and shipped on dry ice to ArrayStar (Rockville, MD, USA) for mRNA processing, microarray hybridization, and probe expression normalization.
Microarray Hybridization and Original Data Normalization.
Microarray hybridization and washing was conducted according to manufacturer's instructions.
This includes the detailed procedures for ChIP and library generation for microarray hybridization and deep sequencing.
The microarray hybridization and data normalization analysis were performed by CapitalBio Corp [19].
Probe synthesis, microarray hybridization and data acquisition were performed as described previously [31], [73].
Sample preparation, microarray hybridization and processing were as previously described by our group [18].
Microarray hybridization and processing, image scanning and processing were performed as previously described [18].
In the initial analysis, microarray hybridization and detection underwent rigorous reproducibility tests (data not shown).
We would like to thank NYU Cancer Institute Genomics Facility for microarray hybridization and scanning.
