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In addition, the OliD program displays the location of cross-hybridizing regions as this information might be useful during the microarray hybridization analysis.
In this study, RNA from MS chronic active and MS acute lesions was extracted, and compared with patient matched normal white matter by fluorescent cDNA microarray hybridization analysis.
If a large number of different point mutations are sought, as is often the case, the most appropriate technology may be microarray hybridization analysis, which can test for tens to hundreds of thousands of different point mutations in the same sample simultaneously.
Competitive dual-color microarray hybridization analysis -including dye swap- was performed using the Mytarray V1.1 platform [49].
KK, LL, WCN and DSA performed the microarray hybridization analysis.
MB carried out microarray hybridization analysis and participated in the drafting of the manuscript.
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We thank Alf Blomqvist for help with breeding and care of the birds; Anna Westring, Lina Strömstedt, Carolyn Fitzimmons, Sojeong Ka and Åsa Schippert for laboratory help with the RNA isolation, microarray hybridization and analysis and real time RT-PCR analysis; Leif Andersson and Richard Kirkden for comments on the paper.
WGS, WT, BV and KC participated in the design of experiments, microarray hybridization, microarray analysis, qRT-PCR validation of array results, bioinformatic analysis, data interpretation and manuscript preparation.
This data was originally collected for an analysis of dosage compensation, and further details about study design, tissue preparation, RNA isolation, and microarray hybridization and analysis have been previously published [ 17].
The microarray hybridization and analysis were performed by Cogenics (Morrisville, NC) according to the manufacturer's protocol (Affymetrix, Santa Clara, CA).
Microarray hybridization and analysis was completed at the University of North Carolina at Chapel Hill Functional Genomics Core Facility.
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