LS conducted the microarray hybridizations, analysed the microarray data, wrote the manuscript, assisted in probe design for the pan- Neisseria microarray, and produced the pan- Neisseria microarray-v2 PCR product probes.
TMC performed the RNA extractions, microarray hybridizations and analyses and drafted the manuscript.
She also carried out the sample preparations, DNA microarray hybridizations, data analyses, and manuscript preparation.
Microarray hybridization and data analyses were performed by the Gene Expression Core of the Interdisciplinary Center for Biotechnology Research at the University of Florida.
Recently, many microarray and substractive hybridization analyses of the gene expression profiles in several human cancers have identified endocan as being one of the genes defining a tumor signature, associated with poor prognosis with an increased risk of death or invasive disease in melanoma, breast, kidney prostate and lung cancers, [ 18- 28, 28, 30].
Fish experiments, design of the rainbow trout cDNA microarray, hybridization protocol and data analyses are described in detail elsewhere [ 10, 11].
As revealed by microarray hybridization and RT-qPCR analyses, AmelCPF1 did not fit the typical pattern of CP gene regulation because its expression is higher in the integument of newly-ecdysed-pupae than in pupae-in-apolysis and pharate-adults.
Resulting offspring were used for all microarray and in situ hybridization analyses; spt+/+ embryos ("wild-type," hereafter) were used as controls for comparison with spt b104/b104 embryos (" spt" hereafter) in the microarray analyses, and both spt +/+ and sptb104/+ were used as "wild-type" controls for in situ hybridization analyses.
This is similar to the Wolbachia core genome size of 621 genes predicted by Ishmael et al. [ 16] by extrapolation from microarray-based comparative genome hybridization analyses of A-group Wolbachia strains.
