Sentence examples for microarray hybridisations were from inspiring English sources
Microarray hybridisations were performed for each of the five basal and luminal samples, hybridising each against universal reference cDNA.
Microarray hybridisations were conducted as previously described [67] with 5 µg Cy5-labelled cDNA derived from amplified M. tuberculosis RNA against 2 µg Cy3-labelled M. tuberculosis H37Rv genomic DNA.
Altogether, 18 microarray hybridisations were performed.
Labeling of the amplified cRNA and microarray hybridisations were performed as previously described [ 25].
Microarray hybridisations were performed in SureHyb hybridisation chambers in a DNA Microarray Hybridisation Oven (Agilent Technologies).
Microarray hybridisations were performed by the VIB MicroArrays Facility (http://www.microarrays.be).be
DNA used in the microarray hybridisations was extracted from 5 ml of an overnight culture (18 h) of MRSA252 using the Edge Biosystems Bacterial Genomic DNA purification kit according to manufacturer's instructions.
The general experimental design for the microarray hybridisations is shown in Figure 5.
A loop design, in which consecutive moult stages were compared via dual channel microarray hybridisations, was employed for the analysis.
Total RNA for use in the microarray hybridisations was extracted as described for the cDNA library construction above.
Amplified and fluorescently labelled cRNA (complimentary RNA) for microarray hybridisations was prepared in accordance with the Two-Color Microarray-Based Gene Expression Analysis - Low Input Quick Amp Labelling Protocol v6.6 (Agilent Technologies, USA).
