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The correlation coefficients calculated from microarray hybridisations ranged from 0.95 to 0.99 indicating that the assays were highly comparable.
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Microarray hybridisations of 34 ovarian cell lines were performed using the Agilent Human 1A V2 array.
While even a simple 2×2 CombiScan required 12 microarray hybridisations, the effort generated output that was highly interpretable.
For microarray hybridisations, DNA was subjected to PCR amplification of the 16S rRNA gene and Cy5-labeled.
LW performed all microarray hybridisations.
HK carried out microarray hybridisations.
Microarray hybridisations were performed in SureHyb hybridisation chambers in a DNA Microarray Hybridisation Oven (Agilent Technologies).
JBGH and BK carried out microarray hybridisations.
Altogether, 18 microarray hybridisations were performed.
Microarray hybridisations were performed in a Lucidea semi-automated system (GE Healthcare), without a pre-hybridisation step.
Microarray hybridisations were performed by the VIB MicroArrays Facility (http://www.microarrays.be).be
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