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In summary, the variability of the generalised log ratios of the 49 microarray hybridisations decreased from (1) total vs. aRNA, (2) total vs. total RNA to (3) aRNA vs. aRNA.
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DNA microarray hybridisations revealed 49 genes with significantly increased and 48 with decreased transcript levels in presence of the native CysR compared to a cysR deletion mutant.
While even a simple 2×2 CombiScan required 12 microarray hybridisations, the effort generated output that was highly interpretable.
For microarray hybridisations, DNA was subjected to PCR amplification of the 16S rRNA gene and Cy5-labeled.
LW performed all microarray hybridisations.
HK carried out microarray hybridisations.
Microarray hybridisations were performed in SureHyb hybridisation chambers in a DNA Microarray Hybridisation Oven (Agilent Technologies).
JBGH and BK carried out microarray hybridisations.
Altogether, 18 microarray hybridisations were performed.
Microarray hybridisations were performed in a Lucidea semi-automated system (GE Healthcare), without a pre-hybridisation step.
Microarray hybridisations were performed by the VIB MicroArrays Facility (http://www.microarrays.be).be
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