Sentence examples for microarray hybridisation we from inspiring English sources
To obtain RNA for microarray hybridisation, we used the following procedure.
To generate fluorescently labelled samples for microarray hybridisation, we used a direct labelling protocol.
For microarray hybridisation we used a common reference design as proposed by Dudley et al. [ 68], in which a fluorescence labelled antisense oligonucleotide (complementary to a sequence tag present in all spotted microarray probes) is hybridised together with the labelled cDNA of interest.
Using a set of 20 microarray hybridisations, we have made a significant contribution to understand the temporal transcriptional response of bovine PBMC in vivo to a controlled trypanosome infection.
Using a publicly available gene expression dataset derived from microarray hybridisations we wanted to find out if imprinted genes form a subset of genes expressed in a particular fashion in human and mouse.
In order to corroborate the results of the microarray hybridisation analysis, we randomly selected 116 ORFs, with 66 of these being included in the variable ones (P < 0.001) and 52 found to show no significant difference compared to the type strain (table 3).
We used the cDNA synthesis, amplification, microarray hybridisation and washing protocols supplied by Agilent (Agilent Technologies, Waldbronn, Germany) with no modifications.
We here present a study of global gene expression using microarray hybridisation in a collection of anal carcinoma biopsies.
The central assumption of this study is that heterologous microarray hybridisation can be used to generate useful data with probes from closely related species.
To test the validity of signal quantitation by the Pinus microarray, 60-mer probes were selected and corresponding transcripts in cDNA transcribed from the original total RNA extracts used for microarray hybridisation were quantified by RT-PCR.
Further details on the microarray hybridisation are available at the MRC/CSC/Imperial College Microarray Centre website (http://microarray.csc.mrc.ac.uk).csc.mrc.ac.uk
