Sentence examples for microarray hybridisation intensity from inspiring English sources

For this analysis, we compared log2 reads per kilobase (of transcript sequence) per million reads (RPKM) with the log2 raw microarray hybridisation intensity probe signals.

For each gene, the microarray hybridisation intensity was plotted against progression-free survival of each patient and linear and quadratic regression equations were computed.

They highlight the significant effect that short sequence mismatches can have upon microarray hybridisation intensities even for long oligonucleotide probes.

However, it is difficult to quantify expression of low-abundant mRNA transcripts by microarrays, since low hybridisation intensity signals are difficult to distinguish from background levels arising from non-specific hybridisation.

All 7 ORFs showed more than one band in the DNA of wild strain compared to the single band of strain Rm 1021, confirming that the higher intensity of the microarray hybridisation of the wild strain was indeed due to a duplication of the ORF.

Great variation in signal intensities commonly affects DNA microarray hybridisation experiments (e.g., [68] [72]).

Multi-experiment SAM and the subsequent iterative hierarchical clustering-support tree analysis (HCL-ST) were carried out with TIGR's MultiExperiment Viewer 4.3 (MEV) by introducing normalised microarray hybridisation data matrixes (including medians and standard deviations of intensity and F values) of clones with significant differential regulation in each individual experiment.

A greater level of probe mismatching is tolerated within a microarray hybridisation (1 6 base differences over 60-mer probe can reduce signal intensity by at least 50% (LeProust [2008]; Hughes et al. [2001])) compared with lower levels of mismatch that are permissible within a quantitative PCR experimental design.

Probe-pairs from the gDNA.cel files were selected using a.cel file parser script [ 31] which produces a probe-mask file (.cdf) compatible with a range of microarray analysis packages and containing only probe-pairs in which the perfect-match probe has a gDNA hybridisation intensity greater than the user defined gDNA hybridisation threshold [ 22].

The central assumption of this study is that heterologous microarray hybridisation can be used to generate useful data with probes from closely related species.

To test the validity of signal quantitation by the Pinus microarray, 60-mer probes were selected and corresponding transcripts in cDNA transcribed from the original total RNA extracts used for microarray hybridisation were quantified by RT-PCR.