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Microarray "gene chip" technology -- silicon chips coated with DNA fragments -- and a software program designed by university experts enabled the researchers to do simultaneous monitoring of about 6,800 genes eight times over three days to see whether they were active or inactive.
"He combines a broad knowledge of science with amazing communication skills and an ability to translate his research discoveries into practical applications". The highest profile product of his work, the microarray "gene chip," took root during his 1989 leave of absence from Stanford to help found Affymax, a therapeutic drug discovery company in Palo Alto.
The microarray gene chip platform is a powerful tool allowing for the analysis of thousands of genes simultaneously.
At the same time, microarray (gene chip) data have been collected that have substantially longer follow up times and extensive clinical annotations.
We used a custom microarray gene chip to characterize gene expression responses of axolotls (Ambystoma mexicanum) to an emerging viral pathogen, Ambystoma tigrinum virus (ATV).
However, the conventional microarray (gene chip), with the ability to examine tens of thousands of genes are now being replaced by a second generation of arrays that are more focused, application-specific and lower-cost.
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Gene expression analysis in 10 μg of total RNA was performed using microarray gene chips (Human Genome U133 Plus 2.0 Arrays; Affymetrix).
Data from all 19 microarray gene chips generated a correlation value (i.e. PCA value) of 68.9% with two distinct outliers.
Affymetrix U133A and U133B microarray gene chips were used to measure gene expression levels from RNA samples.
To identify genes affected early by the HDIs, we differentiated and treated MC3T3-E1 cells with three HDIs or vehicle for 18 hours and analyzed gene expression profiles with Affymetrix microarray gene chips.
Gene expression microarrays (gene chips) have revolutionized biology by generating vast amounts of data roughly quantifying the level of mRNA expression for thousands of genes in a single sample.
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