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It is also important to consider that if the intended clinical assay is different from the high throughput platform, validation of the new assay (eg, PCR or immunochemistry) vs the original microarray findings is necessary and inter- and intra-assay variation should also be provided.
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Our microarray findings were confirmed by quantitative realtime PCR on patient samples as well as on samples from healthy normal controls that underwent the TI procedure.
Microarray findings were validated by real-time quantitative PCR.
The microarray findings were validated using quantitative real-time RT-PCR.
Microarray findings were then corroborated and extended by real time RT-PCR in the same cohorts of subjects, as well as in an independent group of 25 MS patients and 11 healthy controls.
HELP microarray data has been submitted to the GEO database for public access (accession number pending) Validation of HELP microarray findings was carried out by MALDI-TOF mass spectrometry using EpiTyper by MassArray (Sequenom, CA) on bisulfite-converted DNA as previously described[31].
Hence, the microarray findings were validated.
In all cases, the microarray findings were confirmed.
Microarray findings were validated with real time RT-PCR analyses.
Key microarray findings were verified by real-time PCR and additional in vitro experiments of matrix synthesis and signal transduction.
Microarray findings were further validated by qRT-PCR analysis in another bioptic series of 75 mucosa samples.
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