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Finally, RT-PCR analysis of many genes on an independent RNA set confirmed microarray findings (described below).
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The findings described in the G.A.O.
One report has described microarray findings in MS brain lesions using autopsy samples from human subjects [ 8].
Hence, the microarray findings were validated.
Next, we further verified the microarray findings.
In all cases, the microarray findings were confirmed.
We prospectively validated the microarray findings in independent samples.
Microarray findings were validated with real time RT-PCR analyses.
The PCR results were consistent with the microarray findings.
RNA was extracted and processed for microarray as described above.
The microarray application described here is unlike most array assays.
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