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Thus, our dissections produced prominence specific material, and there was an excellent correspondence between our microarray findings and established differential gene expression patterns.
Over-expression and under-expression ratios were statistically consistent with the microarray findings and the overall trend of gene expression was similar for both microarray and qRT-PCR experiments for all the tested genes (Table 6), as shown by Pearson correlation (data not shown).
These genes were selected to confirm the microarray findings and because of the functional annotation of their encoded proteins.
These results confirm microarray findings and demonstrate that such a diversity is indeed an intrinsic characteristic associated to the tumor or normal tissue origin of the EC, regardless of the culture conditions.
To validate our microarray findings and provide insight into the mechanism of TNF-induced VEGF-C expression in OCPs, we provide preliminary evidence indicating that this response is mediated by NF-κB-dependent transcription.
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Consistent with the microarray findings, Etv1 and Prox1 exhibited enteroendocrine specificity and were found at significantly lower levels in upper SI K- than upper SI and colonic L-cells.
It is also important to consider that if the intended clinical assay is different from the high throughput platform, validation of the new assay (eg, PCR or immunochemistry) vs the original microarray findings is necessary and inter- and intra-assay variation should also be provided.
Quantitative real-time-PCR confirmed microarray findings for miR-1183 and miR-1299 in both tissue and plasma.
The focus of the discussion and conclusions should be on the microarray findings on proteases and the wealth of functional data on proteases presented in the manuscript.
These data are consistent with the microarray findings (Tables 1 and 2), which indicate GIPC1 silencing inhibits cell proliferation and promotes apoptosis.
Focusing on the genes previously suggested to be involved in the pathophysiology of depression, we were able to confirm small but significant expression differences that were in-line with the microarray findings (Tables 1 and 2).
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