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For analysis of microarray data from DHA experiments, the.gpr microarray files were used as input.
All associated microarray files were uploaded to ArrayExpress [ 83] under the accession numbers A-MEXP-2380 A-MEXP-2380 A-MEXP-2380B-2341 (protocol andesign file).
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Microarray files are read in R using BioConductor.
If the experimental record in the ArrayExpress database contains all of the data necessary for the system to process it and the microarray files are of a type recognized by the platform, the experiment is downloaded, and the platform notifies the user when the download process is complete.
The oligonucleotide probe identifiers and signal intensities from the scanned microarray image files were determined using software running on the microarray scanner (Feature Extraction, v 10.5.1.1, Agilent).
The microarray GPR files were analyzed using the linear models for statistical analysis of microarray data (LIMMA, version 3.4.5) software [ 36] package in R (version 2.11.1) [ 37].
Microarray image files were analysed with GenePix Pro 4.1 (Molecular Devices, Sunnyvale, CA, USA) and loaded into the UNC-CH Microarray Database (https://genome.unc.edu/), and are available in the Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo) under the series numbers GSE8465.
Microarray summarization files were analyzed using the Bioconductor in the R framework.
The scanned microarray image files were then loaded into Agilent Feature Extraction Software (v. 9.5.1), which was used to perform calculations that included feature intensities, background measurements, and statistical analyses.
The 44 scanned microarray image files were uploaded to the feature extraction software.
Microarray data files were downloaded from the Gene Expression Omnibus (GEO) database [ 32].
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