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Technical replicas were present in each slide (see Microarray features) and they were split in two subarrays to check the quality of microarray data, as explained in Supporting information (Text S1).
NCBI UniGene build #230 was used to assign gene annotation data to microarray features and match data between platforms.
The basis of all cross-platform comparison is reliability of annotation and mapping of microarray features and tag sequences, especially if transcripts from the sense or antisense orientation are to be distinguished.
As noted at the caret documentation, the colinearity filter computes the correlations between the microarray features and then screens the correlation matrix, in order to remove features with high pairwise correlation.
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Briefly, after scanning of the DNA microarrays, features and local background intensities were detected and quantified with GenePix 4.0 software (Molecular Devices).
Overall, from this work it emerged that after 4 days of exposure to 0.01 μg/L BPA, approximately 4% and 2% of microarray features for fathead minnow and zebrafish females, respectively, had expression levels significantly different from controls.
Their corresponding HTR cluster identifier was used to represent MPSS tags and microarray features.
The competitive Cy5-labeled total cDNA provided clear microarray feature identification and a guide to relative sequence abundance in the pre-purification sample from which the relative expression rank of each mRNA was determined ([see additional file 1, sheet 2]).
We detected the differential expression of 49 microarray features (29 unique annotated genes and one gene with unknown function) associated with protein folding, protein synthesis, metabolism, oxidative stress and ion transport that were common between populations and species of Pacific salmon held at 19°C compared with fish held at a cooler temperature (13 or 14°C).
An R-package to match array comparative genomic hybridization (CGH) and gene expression microarray features for integrative analysis purposes was provided by van Wieringen et al. (2012).
At q < 0.01, 103 microarray features in 2007, 243 features in 2008, and 3541 features in 2009 had significantly different levels of expression between the temperature treatments (Fig. 3).
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