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The particular procedures for microarray fabrication were conducted according to [ 37].
In our setting all clones used for antigen-purification and microarray fabrication were selected via several pre-screens within the SEREX procedure (see methods and additional file 1: detailed information on methodology).
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However, costs of single-species design and microarray fabrication are prohibitive.
The microarray fabrication was carried out in a controlled environment at 38% humidity and the temperature was held constant at 23°C.
Since microarray fabrication is a considerable source of data variance, we previously directly tagged cDNA probes with a third fluorophore for prehybridization quality control.
Thus, false positive-signals derived from carry-over of reactive proteins from printing spots with the same set of pins during microarray fabrication can be excluded.
The one-step, cost-effective microarray fabrication method is applicable to various non-modified plastics and results in both high spot quality and high immobilization efficiency.
Both human miRNA microarray fabrication and hybridization were performed as described previously [ 26, 28].
"This fabrication is called scuba," she said.
"All the kitchen fabrication is done locally.
The fabrication is our innovation.
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