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The microarray fabrication was carried out in a controlled environment at 38% humidity and the temperature was held constant at 23°C.
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However, costs of single-species design and microarray fabrication are prohibitive.
The particular procedures for microarray fabrication were conducted according to [ 37].
Since microarray fabrication is a considerable source of data variance, we previously directly tagged cDNA probes with a third fluorophore for prehybridization quality control.
In our setting all clones used for antigen-purification and microarray fabrication were selected via several pre-screens within the SEREX procedure (see methods and additional file 1: detailed information on methodology).
Thus, false positive-signals derived from carry-over of reactive proteins from printing spots with the same set of pins during microarray fabrication can be excluded.
The one-step, cost-effective microarray fabrication method is applicable to various non-modified plastics and results in both high spot quality and high immobilization efficiency.
Template fabrication was similar to previous work.
Fabrication of FNDs: The FND fabrication was based on the method described previously [ 3, 27].
Device fabrication was largely straightforward using multilayer soft lithography.
Gold microelectrode fabrication was carried out using well-established methods.
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