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The coding gene's result page includes gene annotation, the list of co-expressing genes, 14 microarray expression experiments [only if there is a probe set(s) in its context] and deep sequence evidence of it in the 30 deep sequencing samples.
Microarray expression experiments were performed on 4×44 K Agilent Human and Mouse expression arrays (Agilent technologies) by hybridizing SH-SY5Y cells according to the manufacturer's instructions.
Our coexpression matrices were obtained from publicly available microarray expression experiments analyzed in a microarray meta-analysis system (Gemma, http://www.chibi.ubc.ca/Gemma).ubc.ca/Gemma
The advantages of microarray expression experiments are the maturation of this technology, high-throughput and many types of analysis software.
In microarray expression experiments, a simple fold change of 2 is recommended between two conditions to obtain more reliable results.
In planning microarray expression experiments, this paper highlights a number of concerns that an investigator should consider in defining a new experiment or in comparisons with reported results.
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A typical example could be a cluster of co-expressing genes identified in a microarray expression experiment.
In a typical microarray expression experiment, the relative expression levels in two different biological samples (cDNA arrays) or the absolute expression levels in a single biological sample (oligonucleotide arrays) are measured simultaneously for tens of thousands of genes.
Microarray expression experiment data (CEL files) were downloaded from the Gene Expression Omnibus (GEO) [ 17] in the National Center for Biotechnology Information (NCBI) and normalized using Affymetrix GCOS software (TGT value: 500).
The large body of published biomedical literature is one of the richest data sources for systematically identifying colorectal cancer related genes without microarray expression experiment.
Microarray expression experiment data (CEL files) were downloaded from GEO [ 17] in NCBI and normalized using Affymetrix GCOS software (TGT value: 500).
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