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We regarded those combinations that gave the highest average microarray expression change to be the optimal organization for Met4 coordination.
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The aim of this project was to study the miRNA microarray expression changes in human umbilical vein endothelial cells (HUVEC) treated in a diabetic-like environment with the addition of calcitriol.
The discordance of hTERT gene expression result may be explained by the decreased sensitivity of the microarray gene expression change compared to real-time PCR.
Additionally, QRT-PCR Ct values also show strong fold changes of 28.6 and 16.0 for SPP1 and CENPA, consistently with microarray expression fold-changes (Table 6), strongly suggesting a possibility to consider this gene pair as a prospective biomarker of lung AC.
However, by explicitly accounting for variance over time, a set of 85 genes were identified subsequently in our first 11 VAP patients whose microarray expression levels changed consistently before the clinical diagnosis of VAP.
Quantitative real-time RT-PCR assays were performed to confirm microarray gene expression changes (Table 4).
To confirm the microarray predicted expression changes we performed immunohistochemisty, comparing wild type glomeruli to those of db/ db mice.
We confirmed the authenticity of the microarray-measured expression changes for seven genes within the 203 gene set by quantitative real time polymerase chain reaction (qRT-PCR) (Table 3).
We confirmed the microarray-detected expression changes in two of the chosen genes, Gabra5 and Hspa5, via in situ hybridization (Fig. 1A and B) and the third gene, Syn1, was interrogated by two independent microarray probe sets that exhibited nearly identical results (Fig. 1C).
SplicerAV: a tool for mining microarray expression data for changes in RNA processing.
Ten representative genes were selected from the microarray expression profiles and changes in the expression level confirmed using qPCR.
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