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To compare patterns of selection among genes expressed at distinct life stages, we ran microarray expression assays on seven different life-stages/tissues of S. purpuratus: ovary, testes, 2-week-old pluteus larvae, and 4 adult somatic tissues (coelemocytes, spine base, tube foot, and gut).
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Several commercially available methods for reducing the impact of globin RNA transcripts in the microarray expression assay have been developed.
Microarray gene expression assays have been performed as described previously [30].
Patients with refractory advanced cancer had their tumour samples analysed using immunohistochemistry (IHC), fluorescent in situ hybridization assays, and oligonucleotide microarray gene expression assays.
Extensive, long term usage of microarray gene expression assays in clinical studies has produced a vast repository of extremely valuable, well-designed datasets.
RNA preparation and labeling methods designed to reduce the impact of globin mRNA transcripts can significantly improve the sensitivity of the DNA microarray expression profiling assay for whole blood samples.
As noted by the authors, the microarray-based expression assays also did not detect mRNA levels sufficient for analysis for many additional neuropsychiatric disorder candidate genes.
The nonredundant ESTs generated from this library will allow us to construct cDNA microarrays for expression assays and to localise them onto a physical map for assessing genes that regulate fat accumulation in backfat tissue and further affect meat quality in pigs.
These data can be used to develop microarrays for gene expression assays or serve as a reference transcriptome for future RNA-seq experiments in Pteridium.
Another factor may potentially contribute to the anti-correlation is the difference in probe designs for microarrays and TaqMan Expression Assays.
To date, three major studies have used microarray-based mRNA expression assays combined with genome-wide genotyping to quantify mRNA expression and identify eQTL's for genes expressed in human brain [ 16- 18].
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