Using 3,934 diverse mouse microarray experiments we found striking similarities in transcriptional system regulation between human and mouse.
To validate our microarray experiments, we carried out real time PCR analysis of some of the identified genes.
At each of the time-points at which RNA was obtained for microarray experiments, we quantified the expression of at least one transcript.
Beside the scores of the candidates and the fold-change derived from the microarray experiments, we also present known links to similar diseases with related phenotypes.
To confirm gene expression patterns derived from our microarray experiments, we performed real-time RT-PCR with four selected genes that were down-regulated in the ΔpdeH mutant.
In the first set of microarray experiments, we generated gene expression data from ten lingual epithelium (LE) samples and ten taste bud (TB) samples - six from FG papilla and four from CV papilla.
From our cDNA microarray experiments, we determined that a variety of nucleotide synthesis-related genes, including TS, were downregulated with lapatinib, and this was apparent in HER2-amplified cells.
In order to apply our age predictor across diverse microarray experiments, we needed to address two issues: microarray platform differences and baseline differences attributable to variations in experimental technique.
To analyze the data generated from the microarray experiments we used GeneSpring GX 10.0.2 (Agilent technologies, Inc) software package for the data Quality Control and the statistical analysis of the microarray data.
In a series of DNA microarray experiments, we compared expression profiles of untransformed wildtype AX2 cells and the CbfA-deficient mutant JH.D with both strains constitutively expressing GFP-CbfA724 998. GFP-CbfA724 998
Utilizing daf-2 alleles with distinct orders of phenotypic severity for hypoxic sensitivity versus its other phenotypes as the basis for DNA microarray experiments, we identified 182 genes that were differentially expressed in animals with varying levels of hypoxia resistance.
