Sentence examples for microarray experiments validation from inspiring English sources

As is common practice with microarray experiments validation of gene expression highlighted by the Pinus microarray via quantitative RT-PCR is required; a caveat for validation of heterologous hybridisation is that primer design needs to overcome sequence differences that can exist between the heterologous test probes and the microarray 60-mer sequences.

RS, PS, RM and SW performed microarray experiments, validation of miRNA and mRNA expression levels, and in situ hybridization.

XL carried out cDNA microarray experiments, RT-MLPA validation, microarray and SilviScan data analyses, and manuscript preparation.

One of the most important issues after performing microarray experiments is validation of their results.

These experiments, which provided source material for microarray experiments and validation tests, included short-term (48-h) independently replicated (n = 3) exposures to non-lethal concentrations of cadmium (LC01, 20 μg Cd/L) and control conditions.

In order to cover the range of variation of times points studied in the microarray experiment, the validation of the expression profiles of the genes by qRT-PCR was done with samples obtained at 16hai for five genes and with samples obtained at 48 hai for other 5 genes.

KM carried out construction of the cDNA library and microarray experiments, qRT-PCR validation, and manuscript preparation.

GN designed and carried out the microarray experiments, performed qRT validation, analyzed the data, and wrote the initial draft of the manuscript.

The Daphnia used for microarray experiments and for validation tests by RT-PCR were exposed in repeated experiments in batches of 50 adult organisms plus their offspring per 3.5-l exposure chamber.

CD prepared the samples for microarray experiments and participated in validation of the results.

SF carried out transcriptome sequencing and annotation, probe design, microarray experiments and qRT-PCR validation.