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The relative expression levels for all genes tested were consistent with the results of the microarray experiments, thus confirming its reliability.
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Many ESTs, in particular unannotated ones [ 10], may be expressed under limited environmental conditions that are not represented by this microarray experiment, thus leading to their classification as non-functional.
The results showed that the messenger RNA for the selected genes follows a similar pattern of expression to that observed with the oligo-DNA microarray experiment, thus confirming the results and the quality of the data obtained with the high-density microarrays.
Microarray experiments were thus carried out using a previously described ErbB2-overexpressing HMLEC system [ 31, 32].
Due to the three discarded microarray experiments we thus obtained five knockout and four overexpression scores for each gene.
Batch effects due to sample preparation or array variation (type, charge, and/or platform) may influence the results of microarray experiments and thus mask and/or confound true biological differences.
The combination of results from different studies partially solves the problem of a small sample number (inherent to microarray experiments) and thus helps to detect the truly differentially expressed genes.
The microarray experiments were thus designed to identify genes that are differentially expressed after 24 hours of Al exposure at concentrations resulting in 50% RGI for all lines used.
As shown in Additional file 5: Table S5, the temporal patterns and expression levels of the selected genes were generally consistent between the microarray and qPCR experiments, thus validating the rabbit microarray gene expression data.
Thus, microarray experiments were not included.
Thus, DNA microarray experiments would be extremely helpful to study effects on the transcription of genes involved in these processes, to provide more insight in potential mode of actions of rhodomyrtone.
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