Closely related gene-family members, sharing more than 80% sequence homology, can cross-hybridize in cDNA microarray experiments resulting in false positive results [ 31].
QP participated in the design of the study, analyzed the microarray experiment results, and participated in drafting the manuscript.
Locus identifiers were submitted to the Genevestigator Arabidopsis thaliana microarray database and analysis toolbox [ 27] at, where they were assayed against 1434 developmental and tissue-specific Arabidopsis microarray experiment results [ 35- 38].
The total microarray experiment resulted in 8 hybridizations of 16 unique samples.
These secondary structures may therefore compete with the intended target for effective probe annealing in a microarray experiment, resulting in a misinterpretation of the amount of target present in the sample.
Complete information about the microarray experiments and results can be retrieved from the ArrayExpress database at the European Bioinformatics Institute [ 24] by the following accession number: E-MTAB-761.
BaP concentrations (0.25, 1.00, 2.50, 5.00 μM) were then chosen for the microarray experiments that resulted in low to high DNA adduct formation.
Analysis of a microarray experiment often results in a list of hundreds of disease-associated genes.
This type of analysis applied to the samples of a microarray experiment typically results in clusters of samples with a similar expression pattern, thereby revealing the main factors that lead to changes in gene expression.
A microarray experiment may result in hundreds of differentially expressed genes that are subject to interpretation and further analysis.
