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RDW performed biofilm and planktonic culturing, microarray experiments, quantitative RT-PCR, data analysis and drafted the manuscript.
KB performed bioreactor cultivations, proteomics and microarray experiments, quantitative real-time PCR, data analysis and interpretation of the results, and drafted the manuscript.
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To confirm the results of the microarrays experiments, quantitative reverse transcriptase PCR analysis was performed on seven regulated genes at 4.5 hpf (30% epiboly) that were chosen as hallmarks of the differences between the ERK1 and ERK2 morphant expression profiles.
GZ carried out the confirmatory analysis of microarray experiments through quantitative real time-PCR and various technical assistance and advice.
To confirm the results obtained from the microarray experiment, a quantitative real-time-polymerase chain reaction (qPCR) was performed on 45 patient samples.
We validated the microarray experiments by 352 quantitative real-time PCR tests using the same total RNA samples.
To confirm the results from microarray experiments, real-time quantitative RT-PCR was carried out using the same RNA preparations that were used in the microarray experiments.
To verify the expression of genes identified in microarray experiments, real-time quantitative RT PCR was performed using the same RNA as that used in the microarray analysis.
In all cases, we obtained a strong correlation between the results obtained in microarray experiments and in quantitative real-time RT-PCR.
Batches of cells were infected for microarray experiments, and a quantitative polymerase chain reaction was used to verify the reproducibility of the changes in gene expression.
In order to confirm the observed cDNA-AFLP expression profiles and also to link these to the expression patterns found in the microarray experiments described below, quantitative RT-PCR (q-PCR) experiments were carried out.
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