In the microarray experiment, chaperone-like genes such as HSP90-alpha, HSP90-beta, HSC71, HSP47, peptidyl-prolyl-isomerase A, CCT1 and CCT6 (Table 2) were identified as either being induced by pIC at 24HPI in fish injected at 16°C but not in fish injected at 10°C, or significantly more highly expressed at the mRNA level in the pIC@16°C fish at 24HPI when compared to the pIC@10°C (Table 2).
The preferential transcription detected by genome microarray experiments of mqsR, sirA-like, poxB, acsA, pta, clpX, rpfG-like, ackA and the unknown orf ygiT-like (downstream of mqsR), was confirmed by RT-PCR (Fig. 3).
Just like regular microarray experiments, the observed fluorescence intensities are interpreted as elevated transcription activity at specific genome locations.
Expression data from ESTs and microarray experiments for KLC genes is consistent with the hypothesis that AaKLC2-like genes are zygotic-specific genes and AaKLC1-like genes are ubiquitously expressed genes (Table 2).
A set of genes altered in microarray experiments were validated using a second method like semi quantitative RT-PCR.
We would like to thank Darren Morrow for technical advice on the microarray experiments.
We would like to thank everyone at the Genopolis Consortium for their contribution to microarray experiments and bioinformatics analysis.
We would like to thank Scott Tighe and Tim Hunter for their contribution to the experimental design of the microarray experiments.
We would like to thank Chris den Hengst for constructs, protocols and advice on ChIP experiments, Maureen Bibb and Andy Hesketh for advice on microarray experiments and Jeremy Thornton for technical assistance.
The microarray experiments revealed more than two hundred genes that were unusually active in the placentas of the women suffering from preeclampsia.
