The alternative explanation, that the relevant mRNAs were not detected is unlikely based on the results of the data analysis and experience with highly similar microarray experiments for S. aureus [ 26].
By using the biological replicate number required in microarray experiments for a given power or by determining the power required to detect a gene significantly differentially expressed, given the sample size, or the best multiple-testing method can be chosen.
In contrast, many genes were used in the microarray experiments for data normalization.
Thus, we gathered data concerning 51 different microarray experiments for D. melanogaster respecting the criterion outlined previously.
The visual observation of the clustering results indicates satisfactory reproducibility of microarray experiments for the biological replicates in each treatment group and significant treatment effects of estradiol and SWT, warranting further analyses and interpretation of their treatment effects.
A comprehensive survey of transcriptomes by transcript or its tag sampling, followed by extensive microarray experiments for repeated measurements under various physiological conditions should be able to significantly accelerate de novo analyses and functional annotations of unknown transcriptomes, especially when the genome sequence of the targeted organism is available.
There are plenty of biases, which are introduced to microarray experiments for technical reasons, e.g. by printing probes with a multi-pin head, by different incorporation efficiencies of the fluorescent dyes, or by just different fluorescence intensities of individual probes [15].
EDA is used extensively in DNA microarray experiments for example.
SR has performed microarray experiments for seedling and stress stages.
We produced single microarray experiments for our twenty conditions (five cell types x four epigenetic marks).
Additional file 11: Expression data from microarray experiments for FMN1, JDP2, RNASE1, and TIMP1 at admission.
