Sentence examples for microarray experiments following from inspiring English sources
To gain an understanding of the function of TFII-I in regulating gene expression and CTCF binding genome wide, we conducted microarray experiments following TFII-I knockdown and chromatin immunoprecipitation of CTCF followed by next generation sequencing (ChIP-seq) from the same TFII-I depleted cells.
Using an identical strategy, we performed microarray experiments following a pulse of expression of each Qrr sRNA (Supplementary Figure S1).
Total RNA was isolated and treated as described by [ 21]. cDNA synthesis was performed using the RevertAid-H First Strand cDNA Synthesis Kit (Fermentas) and Oligo-d(T -Primers for quanT -PrimersT-PCR analysis and Random Hexamer Primers for microarray experiments following the manufacturer's instructions.
In order to rationally filter the large volume of data derived from the microarray experiments, the following strategy was employed to select the genes that are characteristic of the renal response to proteinuria: 1 – Identification of genes differentially expressed in the in vitro model of proteinuria by SAM and Limma analysis (described below).
For the microarray experiments the following genotypes were used: Control/wild type - w 1118 /w 1118 ; +/+; +/+ dADA2bL - w/w; +/+; P{dAda2bL-EGFP}dAda2b d842 /P{dAda2bL-EGFP}dAda2b d842 dADA2bS - w/w; P{dAda2bS-EGFP}/P{dAda2bS-EGFP}; dAda2b d842 /dAda2b d842 The gene ontology categories were scored at the NetAffX website according the Affymetrix sample ID number.
We illustrate one intended use of URR for two-color microarray experiments in the following example.
The microarray experiments were carried out following the manufacturer's protocols.
Totally there are 8 hybridization samples for the following microarray experiments.
The microarray experiments were conducted by following the minimum information about a microarray experiment (MIAME) guidelines [ 56].
LB medium, a rich source of iron (~17 μM) [ 26], was used to provide sufficient biomass for the following microarray experiments.
We have consistently observed S100A2 as a positively regulated BRCA1 target gene following several microarray experiments (data not shown).
