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Contrary to the traditional biology approach, where the expression patterns of a handful of genes are studied at a time, microarray experiments enable biologists to study the expression patterns of many genes simultaneously from gene expression profile data and decipher the underlying hidden biological mechanism from the observed gene expression changes.
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GENEVESTIGATOR is an online platform based on a high quality, manually curated database of microarray experiments enabling gene expression and regulation studies as well as the search for groups of genes sharing similar expression patterns by means of clustering and biclustering algorithms.
The more specialized PathoPlant database on plant-pathogen interactions and components of signal transduction pathways related to plant pathogenesis also harbors gene expression data from Arabidopsis thaliana microarray experiments to enable searching for specific genes regulated upon pathogen infection or elicitor treatment [ 34, 35].
Microarray experiments have enabled the detection of significant variation in mRNA abundance and improved the understanding of the molecular mechanism of partial defense responses [ 6- 9].
This physiological effect was detectable on a global scale by DNA microarray experiments and enabled the screening for differentially expressed genes that were later on assigned to the DtxR regulon of C. glutamicum.
Analysis of the large number of microarray gene expression experiments enabled the first comprehensive characterization of the role of prohormone and prohormone convertase genes in biological processes in the pig.
DNA adducts were measured in cells exposed to BaP for up to 48 h in order to establish biologically significant concentrations to be used for the microarray experiments and to enable gene expression changes to be related to DNA damage levels.
A crucial aspect of all microarray experiments is good system reproducibility enabling direct and reliable comparisons between different experiments.
vRNA in combination with reference designs enable systems biology microarray experiments of small physiologically relevant changes.
High-throughput microarray experiments have modernized biological experiments by enabling measurements of expression levels for genes on the genome scale under different conditions.
Mehdi et al. [ 13] implement interwoven design to improve microarray experiments to ensure that the resulting data enables robust statistical analysis.
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