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RNAlater has been used successfully with C. jejuni [57], and in our hands these storage conditions did not alter C. jejuni NCTC11168 gene expression in control microarray experiments (data not shown).
CB performed microarray experiments, data analysis and drafted the manuscript.
The integrated differential gene expression toolkits delivered similar results as corresponding microarray experiments (data not shown).
JCK carried out the microarray experiments, data processing, and pathway analysis, and drafted the manuscript.
JCK carried out the microarray experiments, data processing, pathway analysis and drafted the manuscript.
VM carried out the isolation of RNA, microarray experiments, data analysis and drafted the manuscript.
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For instance, a workflow could be used to integrate microarray experiment data with quantitative trait loci to filter for relevant genes [20].
For this purpose, relevant genome-wide microarray experiment data (e.g., disease sample vs. reference sample) is required from which the differential expression level for each gene in the genome is computed.
Microarray experiment data E-MTAB-1057 E-MTAB-1057 E-MTAB-1057EBI Array Express Archive [ 107].
YSH performed the microarray experiment, data analysis, and helped draft the manuscript.
Quantitative real-time PCR was used to validate microarray experiment data.
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