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LV developed optimized protocols, prepared the Actichip microarrays, carried out microarray experiments, contributed to oligonucleotide design and data evaluation and drafted the manuscript.
KIP performed the search for prohormone and prohormone convertase genes in the UniProt, Gene, UniGene and ENSEMBL databases, identified the gene probes on the microarray platform, analyzed 35 microarray experiments, contributed to the interpretation of results, and drafted the manuscript.
TL performed the microarray experiments, contributed to the microarray, qPCR and statistical analysis, and is responsible for the preparation of the figures and the final formatting of the manuscript.
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GSB contributed to the experimental development of Bisulfite-tag, carried out gene expression and Bisulfite-tag microarray experiments and contributed to PCR amplicon design.
VM carried out the microarray experiments and contributed to draft the manuscript.
AJ performed part of microarray experiments and contributed to the writing process.
GB amplified the array elements, developed optimised protocols, performed most of the microarray experiments, and contributed to data evaluation.
BK and ETO defined the research theme; BK and CK conceived and designed the methods and experiments, CK carried out the laboratory experiments and performed the data analysis, SE carried out the microarray experiments, KYA contributed by providing a methodological perspective.
In order to identify the parameters of DNA microarray experiments that contribute to the experimental error variance, Figure 4 relates experimental error variance to characteristics of the reporter spot.
SS performed the microarray experiment and contributed to the overall manuscript.
JJS participated in rearing the animals associated with the microarray experiment and contributed to the statistical analyses that were conducted.
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