Based on the preliminary observations from microarray studies and the relation of DHRS7 to SDRs involved in PCa, we investigated the role of DHRS7 in PCa, taking advantage of a combination of human data obtained on a large cohort (n = 348) of samples on tissue microarrays [20,21] and in vitro experiments comprising modulation of DHRS7 expression by knockdown.
Currently GiSAO.db stores gene expression values of 11 experiments comprising 111 Affymetrix microarrays of three different species: Homo sapiens, Mus musculus and Saccharomyces cerevisae.
The data obtained from NASCArrays, from 72 experiments comprising of 956 Affymetrix ATH1 microarray slides, had already been normalized with the Affymetrix MAS 5.0 algorithm to the common mean value of 100.
This comparative study will be useful to scientists needing to choose an amplification method to carry out microarray experiments involving samples comprising only a few cells and generating picogram amounts of RNA.
For the total experiment comprising 15 microarray groups, each composed of a pool of 12 mice, analyzed in triplicate, 4649 quantile-normalized genes were selected for further analysis.
Microarray data were organized in gene expression panels, each composed of M experiments, each experiment comprising N samples.
A total of 19 data sets comprising 278 microarray experiments were compiled based on these criteria.
Since the microarray experiments performed were set up to comprise conditions of active starch biosynthesis, it was believed that comparative analysis of transcription profiles would select for genes involved in this process.
First, we computed the PCCs for all pairwise relationships between the 112 OsSTA genes in two sets of transcriptomes comprising a total of 190 microarray experiments by R-2.14.1 R-2.14.1
Briefly, the experiment comprised of six microarray hybridisations with two triplicates: one for proliferating myoblasts and another for differentiated myotubes.
