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Comparing the types of microarray experiments carried out in these two species shows that there is little overlap [ 6].
GB conducted all of the microarray experiments, carried out the data extraction and performed the pathway interaction analysis.
TB participated in study design and coordination, participated in design, experimental work, analysis and interpretation of microarray experiments, carried out cell and molecular studies and drafted the manuscript.
CSF participated in design and interpretation of microarray experiments, carried out cell and molecular studies and helped to draft the manuscript.
Interestingly, several of the protein changes we have identified in the current study correspond to data presented in reports on previous microarray experiments carried out on either whole brain or cortical tissue from mouse models of Batten disease (38, 39).
In our approach, we are extrapolating results from a variety of microarray experiments carried out within a specific biological context (e.g. tissue type) to predict gene stability in similar contexts.
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To elucidate it further, we first analyzed the distribution of HBS along the 10 Kb upstream promoters of the genes that were differentially expressed in a microarray experiment carried out with HD patients [ 10] (see materials and methods for details).
ECB performed the microarray CGH experiments, carried out the sequence and microarray data analyses and prepared the manuscript.
JC carried out the molecular studies and microarray experiments, HY carried out the analysis on GSEA and provided help with bioinformatics, LPKA conceived of the study, coordinated study, revised the manuscript.
Microarray experiments were carried out using 1-color hybridizations on human miRNA Microarray (V3, 8x15K) (Agilent, Cat No.:G4471A-021827), One glass slide formatted with eight high-definition 15 K arrays, based on Sanger miRbase (release 12.0), Probes: 866 human and 89 human viral miRNAs represented.
Two separate microarray experiments were carried out each for cross-bred and Tharparkar cattle.
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