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Based on this observation, we start by identifying activated TFs from a two-condition microarray experimental dataset (Section 2.1).
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SoyKB has many microarray experimental datasets for public access under 99 stress conditions and 25 tissue types acquired from NCBI GEO [ 16] and Array Express [ 17], in addition to 7 leaf and root tissue types and time-course data generated by our collaborators, currently only available for private access.
The mean for each transcript was determined across all microarrays for the given experimental dataset.
In this study, we compared nCounter with microarray expression measurements of an experimental dataset composed of leukocyte subset RNA from healthy controls and patients with either anti-neutrophil-cytoplasmic-antibody-associated vasculitis (AAV) or inflammatory bowel disease (IBD) to exemplify the level of biological variation likely to be encountered in microarray experiments.
We integrated a compendium of A. thaliana genomic data (55 microarray and 5 interaction datasets) using a Bayesian framework [ 10, 11] to probabilistic weight each experimental dataset according to its relevance in diverse biological areas.
Figure 6 Experimental dataset.
The classes one-color microarray experimental process and two-color microarray experimental process also specialize DNA microarray experimental process.
For the microarray experimental design, RNAs from consecutive time-points were hybridised on the same arrays in a two-channel loop-design (design and complete dataset available at ArrayExpress [ 21] accession E-TABM-807).
Spurious correlations between adjacent and periodically spaced genes were previously identified by gene-gene correlations across experimental datasets encompassing multiple microarrays [ 21- 23].
Finally, to display other experimental datasets (e.g. microarray, RNA-Seq) it now has support for user-defined input types, which can be rendered graphically as either graphs or heat maps.
Additional experimental datasets can be obtained from the microarray repositories and added to the pipeline by the authors as they become available.
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