Exact(4)
Highly variable microarray experimental conditions and the use of different microarray platforms cause poor reproducibility of microarray measurements within and between laboratories [7] [11].
Thus, we do not expect biases in the microarray experimental conditions to severely influence the correlations in expression context.
The obtained IC50 was then further confirmed in 50-ml large-scale cultures as previously described in order to closely mimic the microarray experimental conditions [ 18].
For example, for a TRN that has the properties shown in Fig. 2, even when we eliminate 50% of the TRN to create a "preliminary TRN", 90% of the constructed TF activities have a Pearson correlation coefficient of at least 0.70 with the TF activities used to generate the synthetic expression data (when 20 or more microarray experimental conditions were used).
Similar(56)
Since our approach using optimal principal components was dependent on data structure, such as microarray platform and experimental conditions, we could not directly apply coefficients obtained from our microarray dataset to the other independent microarray datasets.
Heterogeneity of patients in terms of clinicopathological characteristics as well as differences in microarray platforms and experimental conditions are among the main causes of inconsistent microarray results among datasets.
Table 1 shows correlations between the phenanthrene microarray and other experimental conditions.
47 This holds all of the microarray data, the experimental conditions, and information on saccharide probes and proteins.
This database contains microarray data of 40 different experimental conditions obtained through microarray analysis using the same custom microarray chip as described in this manuscript.
In case of non-availability of the microarray data for the identical experimental conditions being analysed, microarray experiments representing broadly similar conditions may be used for the analysis.
Considering the high sensitivity of the microarray approach, and various experimental conditions and arrays that were used, it is not surprising that all these microarray profiles, including ours, are not comparable to each other.
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