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Biotin-labeled target for the microarray experiment were prepared using 1µg of total RNA.
For that, 6 of the 10 RNA samples (3 of 5 from each growth condition) used for the DNA microarray experiment were applied to RT-PCR.
Equal amount of RNA samples from all the four biological replicates used in microarray experiment were pooled together for electrophoretic analysis.
Similarly, 19 genes that were downregulated in our microarray experiment were found to be common to Rubin's list of genes essential for in vitro growth of mycobacteria.
The differential regulations observed in the microarray experiment were confirmed for ADORA1 (EAT vs MAT), ADRA2A, LIPE and PTGDS (Figure 4).
Equal amount of RNA samples from all the four biological replicates used in microarray experiment were pooled together for use in RT-PCR.
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Each microarray experiment was performed in duplicate.
Raw data of microarray experiment is provided in Table S5.
Microarray experiment was commercially done by Agilant Genotypic Technology, Bangalore, India.
The microarray experiment was carried out as described [46] with minor modifications.
Whole transcript microarray experiment was performed.
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