Microarray experiment was performed between LPS-treated and control samples using the Agilent one-color bovine v2 bovine (v2) 4x44K array slides.
Each microarray experiment was performed in duplicate.
The microarray experiment was carried out as described [46] with minor modifications.
A microarray experiment was performed using the Maize Oligonucleotide Array (www.maizearray.org).org
Microarray experiment was commercially done by Agilant Genotypic Technology, Bangalore, India.
The differential expression pattern observed in the microarray experiment was evaluated for 16 genes using qRT-PCR (Figure S1).
The average age of subjects in the microarray experiment was 66 years (range 48 to 76 years).
The main microarray experiment was performed using the Illumina iScan, which is based upon fluorescence detection of biotin-labeled cRNA.
The biological reproducibility of the microarray experiment was determined and the pair-wise scatter plots are shown in Figure S2.
The microarray experiment was set up as a reference design, using a RNA pool of all individuals in each generation as reference.
Another microarray experiment was performed using hepatic RNA from 3 mice with dietary iron overload and 2 mice fed a standard diet.
