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In the microarray experiment, the total number of probe sets detected were 57,381 out of which 43,738 probe sets filtered out and passed the quality check after data pre-processing and normalization.
While considering the design of a microarray experiment, the sources of biological and technical variability must be considered as they affect the ability to detect true gene expression changes.
Basing on the microarray experiment (the datasets that we chose from GEO were from good quality and already normalized) the fold change between the two conditions can be computed for each gene.
In the miRNA microarray experiment, the lymphoblastoid cell lines were grown similarly as above, but without drug treatments.
Specifically, in a DNA microarray experiment, the process of hybridization occurs between a DNA fragment and a cDNA molecule.
Fold induction could not be estimated for miR-513c in the microarray experiment: the signal was detectable upon induction, but was undetectable in uninduced control.
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Next, we analyzed by microarray experiments the IFN-λ-induced gene expression in hepatic cells.
SNH participated in the microarray experiments, the microarray data analysis and the sequence analysis.
In contrast to the microarray experiments, the template RNA was not amplified before cDNA synthesis.
As observed in microarray experiments, the p21 transcript was induced by lovastatin (Supplementary Table 3).
For NASBA-microarray experiment, the 25 best performing probes were selected and additional control probes specific to S.pyogenes, S.agalactiae, M.catarrhalis and K.pneumoniae (three for each) were also added.
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