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The second reference gene was a gene encoding DNA Polymerase IV, which was used in Rhodococcus sp. RHA1, Rhodococcus jostii, and Rhodococcus erythropolis, and this choice was justified based on a microarray experiment performed in Rhodococcus sp. RHA119,20.
Furthermore, hybridization values for PrP were investigated in a microarray experiment performed on in vivo skin wounds.
CB carried out the microarray experiment, performed the statistical analysis and drafted the manuscript.
To our knowledge, this is the first microarray experiment performed in this context.
PHE1 was previously identified as overexpressed in fis1/mea mutants in a microarray experiment performed on younger seeds than we used here [ 28].
EHW carried out the animal trial and microarray experiment, performed the statistical analyses of all data except for the microarray experiment, and drafted the manuscript.
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In fact, whole-genome microarray experiments performed under such conditions (YPD medium) yielded few, if any, specific Mediator targets.
In this way, these data are not representative of typical microarray experiments performed in a research setting, such as those involving clinical samples [6], [7].
YV conceived and designed the microarray experiments performed by KB.
OS conducted NASBA and microarray experiments, performed microarray analysis and drafted the manuscript.
BF designed the microarray experiments, performed the data analyses of microarray data and revised the manuscript.
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