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RNA samples from the nitrogen stress microarray experiments detailed above were also used to generate cDNA samples with two biological replicates per condition.
Each isolate was grown as described for the microarray experiments (for details pertaining to growth of Rd KW20 see Whitby et al 2006 [ 25]) and samples taken at 90 and 110 minutes (20 minutes post FeHm supplementation of the FeHm restricted media).
Microarray experiments were compliant with MIAME (Minimum Information About a Microarray Experiment) and both protocol details and raw data have been deposited in ArrayExpress (http://www.ebi.ac.uk/arrayexpress/) under the accession number E-MEXP-1695 E-MEXP-1695 E-MEXP-1695
A total of 10,990 distinct transcripts, represented in 15,208 array positions, were studied in this microarray experiment (see Methods for details).
Details of microarray experiment were published previously [ 10].
Details of microarray experiment have been submitted to ArrayExpress under accession number E-MTAB-2578 E-MTAB-2578 E-MTAB-2578
Details of microarray experiment have been submitted to ArrayExpress under accession number E-MTAB-2578 E-MTAB-2578 E-MTAB-2578ttp://www.ebi.anduk/areaccessibleexperiments/E-MTat-2578/.
In order to understand how Cyanothece 51142 balances gene expression under nitrogen-fixing conditions in consecutive light and dark cycles with altered physiological requirements under constant light conditions, we analyzed data sets from two different microarray experiments, to elucidate details beyond of what has been found in the former studies [ 16- 18].
The details of the microarray experiment and data analysis were described previously [8].
The experiments in the database in total represented 422 arrays and details regarding each microarray experiment can be easily obtained at www.arabidopsis.leeds.ac.uk/act/expinfo.php.ac.uk/act/expinfo.php
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