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For instance, a workflow could be used to integrate microarray experiment data with quantitative trait loci to filter for relevant genes [20].
For this purpose, relevant genome-wide microarray experiment data (e.g., disease sample vs. reference sample) is required from which the differential expression level for each gene in the genome is computed.
We also found a) there are at least three different mRNAs transcribed from this locus (AceView); b) the SLC6A3-mRNA L241781 encodes 11 exons (exon skipping, SliceInfo); and, c) two plausible intron/exon structures for the SLC6A3 mRNA containing 15 and 16 exons (SPLICY database) as evident from microarray experiment data (probeset annotations).
YSH performed the microarray experiment, data analysis, and helped draft the manuscript.
Microarray experiment data E-MTAB-1057 E-MTAB-1057 E-MTAB-1057EBI Array Express Archive [ 107].
Quantitative real-time PCR was used to validate microarray experiment data.
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RNAlater has been used successfully with C. jejuni [57], and in our hands these storage conditions did not alter C. jejuni NCTC11168 gene expression in control microarray experiments (data not shown).
CB performed microarray experiments, data analysis and drafted the manuscript.
The integrated differential gene expression toolkits delivered similar results as corresponding microarray experiments (data not shown).
JCK carried out the microarray experiments, data processing, and pathway analysis, and drafted the manuscript.
JCK carried out the microarray experiments, data processing, pathway analysis and drafted the manuscript.
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