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To elucidate it further, we first analyzed the distribution of HBS along the 10 Kb upstream promoters of the genes that were differentially expressed in a microarray experiment carried out with HD patients [ 10] (see materials and methods for details).
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Comparing the types of microarray experiments carried out in these two species shows that there is little overlap [ 6].
GB conducted all of the microarray experiments, carried out the data extraction and performed the pathway interaction analysis.
TB participated in study design and coordination, participated in design, experimental work, analysis and interpretation of microarray experiments, carried out cell and molecular studies and drafted the manuscript.
CSF participated in design and interpretation of microarray experiments, carried out cell and molecular studies and helped to draft the manuscript.
Amplified aRNAs were used for microarray experiments carried out by hybridization of WJ-MSCs after 4 passages, compared to WJ-MSCs after 12 passages, for a total of 6 experiments (Table 1).
Interestingly, several of the protein changes we have identified in the current study correspond to data presented in reports on previous microarray experiments carried out on either whole brain or cortical tissue from mouse models of Batten disease (38, 39).
In our approach, we are extrapolating results from a variety of microarray experiments carried out within a specific biological context (e.g. tissue type) to predict gene stability in similar contexts.
The microarray experiment was carried out as described [46] with minor modifications.
The microarray experiment was carried out using a two-channel common reference design with two independent RNA extractions from each tissue sample.
Due to the limited amount of totRNA generated from the peripheral monocytes, the microarray experiment was carried out using aRNA.
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