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Exact(7)
To test whether their activation is induced by dehydration or light, we repeated the experiment and also conducted a similar acute dehydration treatment as using in the microarray experiment but under dark condition.
Only one gene, RT1-CE10, was found to be strongly up-regulated in allogeneic skin explants in the microarray experiment but slightly down-regulated in qRT-PCR.
However, the sequence MT014356, showing similarities with 12-oxophytodienoate reductase (OPR2), was up regulated according to the microarray experiment but down regulated according to qRT-PCR.
One of the genes (m2) showed strong (1.7-fold) Europe over-expression in the microarray experiment, but only slight (1.2-fold) Europe over-expression by qRT-PCR.
Cells were cultured in 80 cm2 flasks for the microarray experiment but for the time course experiment cells were cultured in 25 cm2 flasks for 6, 24 or 48 h.
High quality, unbiased and reproducible gene expression data are always desirable in any DNA microarray experiment, but when the aim is to apply transcriptomics for the identification of illicit use of steroid hormones, data quality becomes essential for obvious reasons.
Similar(53)
Of these, five were actually anti-correlated (decreased in abundance at the mRNA level based on microarray experiments, but increased at the protein level; see Figure 1C).
This comparison was done 'qualitatively'- looking for gene products which showed homodirectional changes (i.e. changed in the same direction) in our proteomic dataset and in microarray experiments, but not considering the magnitude of these changes in these different datasets.
One straightforward solution to solve the missing value problem is to repeat the microarray experiments, but that is very costly and inefficient.
Systematic processing noise, which includes batch effects, is very common in microarray experiments but is often ignored despite its potential to confound or compromise experimental results.
Moreover, using an appropriate scoring function, data can in principle be integrated not just from microarray experiments, but from other sources as well.
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