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Within the microarray experiment all selected transcripts showed significantly altered mRNA abundances between females (HS-females > LS-females), but not between male samples (q-values >0.25).
Data represent the fold-changes in gene expression at steady state obtained from the microarray experiment All three genes were induced by TEL-PDGFRB as confirmed by Q-PCR (Table 2).
To adjust systemic biases that are present in microarray experiment, all of samples passed experimental control metrics such as chromosome X shift and mad.1dr with NimbleScan v.2.5.
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To assess the quality of the RNA used for microarray experiments all samples were analysed using an Agilent Bioanalyser 2100.
The metadata and raw data files of microarray experiments (all MIAME compliant) were deposited in a MIAME compliant database, Gene Expression Omnibus (Accession numbers GSM595960 to GSM595977, a total of 18 samples).
Their abundance within our dataset is a consequence of their transcriptional responses to the microarray experiments; all but four of the assembled sequences were differentially regulated on the arrays.
All fold changes are the result of two biological replicates of the microarray experiment, and all observations with low intensity (<50) in both CRY2 normal and CRY2 knockdown populations have been discarded.
In the microarray experiment contrasting all four possible genotypes, nine transcripts were detected as differentially expressed in animals possessing a maternal callipyge allele (CMat/+ and/or C/C).
The miRNA cgr-miR-185-5p was used as an internal control previously (Jadhav et al. 2012), and the mRNA Actr5 showed very stable expression in the microarray experiment across all CHO cell lines used in this study.
This report includes raw gene expression data files from the resulting microarray experiments from all three radiation levels ranging from a lower, typical exposure than an astronaut might see (0.3 Gy) to high, potentially lethal, levels of radiation (3.0 Gy).
Raw data were deposited in the National Center for Biotechnology Information Gene Expression Omnibus in accordance with "minimum information about microarray experiments" guidelines (all data is MIAME compliant) and given accession numbers GSE20721.
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