To examine robustness of this microarray profiling based evidence, we generated another set of F1 male CNS microarrays.
In addition to the microarray evidence presented here for σ regulation of these operons, we know that STM3773 is the bEBP controlling expression of STM3772-3776 and that this operon encodes the components of a phosphotransferase system permease for D-glucosaminic acid and enzymes required for catabolism of this acid sugar [ 40].
Regarding the POSP subset, we found that of 134 proteins supported by microarray evidence in strain M4, approximately 30% were either associated with mRNA levels differing by less than 2-fold between oocysts and tachyzoites (9.7%) or encoded by transcripts whose relative abundance in tachyzoites was elevated, being comprised between the 50th and the 100th percentile (20.8%).
Human macrophage response to L. (Viannia) panamensis: microarray evidence for an early inflammatory response.
Interestingly, microarray evidence also led to the identification of upregulated rRNA in F2 males.
for further discussion of targets of Gcy1 and Pcs60), consistent with the protein microarray evidence that both proteins are RBPs with broader specificity.
In a previous report, DNA microarray evidence for histone gene induction by Vaccinia virus, based on poly-A dependent RNA amplification, was not corroborated by Northern analysis of one of the histone mRNAs [30].
Generally, the most sensitive interactions in a network are targeted for further elucidation but microarray evidence shows that homeostatic regulation of the steroidogenic network is likely maintained by a mildly sensitive interaction.
In our work, network validation was performed according these two AS metrics: the integrated AS value, named Bayesian AS (bAS) represents the amount of scientific evidence of a putative pathway association by taking into account all the biological data sources, and the AS value obtained from microarray evidence exclusively, named Microarray AS (mAS).
This particular irrigation pond circulation pattern of S. Newport and the microarray evidence of subtle genomic differences among the isolates within the two different lineages of S. Newport (II and III) indicated that some source(s) or host(s) that carried S. Newport continuously shed the pathogen into the environment, causing numerous reintroduction events in the ponds.
