In addition to the microarray evidence presented here for σ regulation of these operons, we know that STM3773 is the bEBP controlling expression of STM3772-3776 and that this operon encodes the components of a phosphotransferase system permease for D-glucosaminic acid and enzymes required for catabolism of this acid sugar [ 40].
For inferring functional linkages from DNA microarray evidence, we employ the divide-test-integrate approach [1] for discovering functionally informative cases of mRNA co-expression.
for further discussion of targets of Gcy1 and Pcs60), consistent with the protein microarray evidence that both proteins are RBPs with broader specificity.
Statistically significant overlaps between differentially expressed gene sets derived from independent expression microarrays provide evidence for shared biological functions between the interrogated experiments.
Our microarray data provides evidence for rpoE2-dependent transcription in eight IGRs where sRNA genes are located: SmelA018, SmelA060, SMa_sRNA_126, SMb_sRNA_381, SMb_sRNA_432 (SMb_sRNA_433Mb_sRNA_433 (Smel-B127ov), SMc_sRNA_259, and SMc_sRNA_259.
Our results (Table 4) indicate that for those transcripts where the individual microarrays showed significant evidence for differential expression (PEG10, PLAGL1, SGCE, and IGF2) there was qualitative agreement with the results of qRT-PCR.
The shared ATX1-AtMTM1 dehydration-responding fractidentifiedfied by microarray analysis provided further evidence for a pathway involving an epigenetic regulator and a lipid phosphatase producing PtdIns5P (microarray expression data are deposited in the NCBI series GSE15577).
In summary, the microarray analyses provide direct evidence for the existence of transcripts from ∼21,100 predicted genes.
Microarray analysis provided transcriptomic evidence for N- but not P-limitation in K. brevis.
Conversely, microarray or even EST evidence for locus expression is not certain evidence of a productive mRNA, particularly for data collected from conjugating cells with nongenic transcription.
