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From the results it can be concluded that padlock probe detection using PCR in combination with microarray detection allows positive detection of GMOs and GMO elements in plant tissues.
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However, UV detection allows for simultaneous detection of unmodified products.
Microarray detection and quantification should therefore allow the simultaneous measurement of copy number at a larger number of loci.
Microarray analysis only allows detection of relative changes in gene expression.
With this work, we present the first CGI microarray that allows the detection of differential DNA methylation in CHO cells on a genomic scale.
This modified microarray system allows direct detection of single nucleotide polymorphisms (SNPs) in human genomic DNA samples without the need for template amplification [ 33].
An alternative for accurate, quantitative global expression profiling is serial analysis of gene expression (SAGE; [ 8]), which in contrast to microarray hybridization allows the detection of new transcripts.
Importantly, the higher sensitivity of this approach allowed us to resolve gametocyte-specific transcription in the large number of samples with gametocyte numbers below microarray detection limits.
Others combined PCR analysis with microarray detection.
Microarray detection was performed with serum samples at 1 5 dilution.
Chromosomal microarray analysis (CMA) allows the detection of microdeletions and microduplications that are not routinely seen on karyotyping [ 8].
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